The formula of bioactive fertilizer and its effects on the physical and chemical properties of soil and economic benefits of maize was studied using field test in the Hexi Corridor light irrigation desert soil of Gansu Province. The results showed that the optimum proportion of biological active fertilizer was as follows:functional improving soil agent was 0.12 tons, bacterial manure was 0.09 tons, and biological active fertilizer was 1.20 tons. There was a positive correlation between fertilization quantity and soil porosity, aggregate structure, water contents, saturated water content, botanical traits, the economic characters and yields of maize. In addition, There was a negative correlatation between fertilization quantity and bulk density, pH value. According to regression analysis, the optimal economical application rate was 1.8 t/hm², and the theoretical yield was 6597.24 kg/hm².