Taking the TM images in 2000 and 2010 as data resource, combined with land use change quantity analytical method and GIS and RS technology, the authors analyzed land use changes and its ecological response in Shenmu County of Shaanxi Province from 2000 to 2010 by adopting the comprehensive land using dynamic degree, land use degree and ecosystem services value(ESV). The result showed that the land use patterns transformed quickly and land use degree reduced in Shenmu County from 2000 to 2010, in which the intensity of land use change of cultivated land, grassland, woodland, water area, industrial land, urban construction and unutilized land reached-1.687%, 0.782%, 0.469%,-0.033%, 0.067%, 0.086%, and 0.316%, respectively. Total value of ecosystem services increased 94 million yuan in Shenmu County from 2000 to 2010. Meanwhile, the ESV of woodland and grassland increased by 347 million yuan and 192 million yuan respectively. On the contrary, the ESV of cultivated land decreased by 395 million yuan, and water area and unutilized land had the smallest change. Spatially, total value of ecosystem services in the northern township mainly declined, at the same time, in southern township, it mainly rose.