[Objection] Differences of soil microbial biomass carbon, nitrogen and soil enzyme activities in different patterns of fruit and grass intercropping were compared in order to provide a support for soil fertility melioration and agriculture modernization.[Methods] Top soil layer(0-10 cm) from four patterns of fruit and grass intercropping was sampled in sites of Dashankou comprehensive agriculture demonstration zone, which located at Chaotian watershed of Guilin area. Variance and correlation analyses were used to compare the differences of soil microbial biomass carbon, nitrogen and soil enzyme activities.[Result] Soil total nitrogen, total phosphorus, total potassium and their available components, soil microbial biomass carbon, nitrogen and soil enzyme activities behaved almost similar ranks as:Fortunella margarita+grass >Guicheng No.1+grass >Pyrus spp+grass >control plot. Pattern of Fortunella margarita+grass intercropping had significant effects on soil microbial biomass carbon, nitrogen and soil enzyme activities(p<0.05). This pattern ameliorated soil better than other patterns did. There existed positive correlations between soil microbial biomass carbon, biomass nitrogen and soil enzyme activities.[Conclusion] Fruit and grass intercropping, especially the pattern of Fortunella margarita+grass, had significant effects on soil amelioration in hilly lands in the southern of China.