添加木醋液对沙地土壤微生物生物量和酶活性的影响
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北京林业大学国家级大学生创新训练项目“施用炭醋肥对沙地土壤性质及植物生长的影响”(G201410022001)


Effects of Wood Vinegar on Microbial Biomasses and Enzyme Activity in Sandy Soil
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    摘要:

    [目的] 探讨沙地添加木醋液后土壤微生物生物量和酶活性的变化,为沙地土壤生物学质量的改良提供理论依据。[方法] 采用盆栽植草培养法,以添加自来水为对照,对沙地添加不同稀释倍数(200,150,100,50,20)木醋液后的土壤可溶性有机碳、氮和酚,土壤微生物生物量碳氮以及土壤酶活性进行研究。[结果] 向沙土添加木醋液可以显著降低土壤pH值,显著提高土壤易氧化碳、土壤水溶性碳氮、土壤可溶性酚以及无机氮的含量。在添加木醋液稀释高于50倍范围内,随木醋液稀释倍数降低,土壤微生物生物量碳氮增加以及β-糖苷酶、碱性磷酸酶和脱氢酶活性提高。添加稀释20倍的木醋液,导致土壤微生物生物量碳氮以及β-糖苷酶、碱性磷酸酶和脱氢酶活性有所降低。在高于20的稀释倍数范围内,随着施用木醋液稀释倍数的降低,α-糖苷酶、亮氨酸氨基肽酶、酸性磷酸酶和酚氧化酶的活性有显著增加的趋势。[结论] 添加不同稀释倍数的木醋液会影响沙地土壤微生物生物量和酶活性。

    Abstract:

    [Objective] To study the effect of the wood vinegar on sandy soil microbial biomass and enzyme activity in order to provide the theoretical basis for improving the sandy soil biological quality.[Methods] Compared with the effect of adding tap water on sandy soil, we added different dilution times(200, 150, 100, 50 and 20) of wood vinegar to the soil using pot planting method. Further, the contents of soil dissolved organic carbon, nitrogen and phenol, soil microbial biomass carbon and nitrogen, as well as soil enzyme activities were determined.[Results] Application of wood vinegar to sand soil could significantly decrease soil pH value, increase the contents of readily soil oxidizable carbon, soil dissolved organic carbon, nitrogen and phenol as well as inorganic nitrogen. When the dilution ratio of wood vinegar was greater than 50-fold, the contents of microbial biomass carbon and nitrogen and the activities of β-glucosidase and alkaline phosphatase and dehydrogenase increased with the decrease of dilution ratio of wood vinegar. Compared with the application of 20-fold dilution of wood winegar, the microbial biomass carbon and nitrogen and the activity of β-glucosidase and alkaline phosphatase and dehydrogenase were decreased with the application of 20-fold dilution of wood vinegar. However, the activity of α-glucosidase, leucine aminopeptidase, acid phosphatase and polyphenol oxidase significantly increased with the decrease of dilution ratio of wood vinegar within a certain range(greater than 20-fold dilution) of wood vinegar.[Conclusion] Different concentrations of wood vinegar will affect sandy soil microbial biomass and soil enzyme activity.

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杜薇,朱一波,张晓,耿玉清,林平.添加木醋液对沙地土壤微生物生物量和酶活性的影响[J].水土保持通报,2016,36(3):358-362,368

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  • 收稿日期:2015-04-01
  • 最后修改日期:2015-07-28
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  • 在线发布日期: 2016-07-12
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