[Objective] Study on soil macropores quantification and 3D reconstruction is particularly important as it can provide reliable theoretical data for preferential flow study. It is also of great importance in precipitation induced landslide studies.[Methods] We took two 25 cm×25 cm×50 cm soil cores from vegetated slope in Panhe Town, Yunnan Province. Base on X-ray computed tomography, a series of plane and longitudinal slice images were collected. By images analysis, variations of soil macropores with the increase of soil depth and CT value of three substances(gravel, matrix, macropore) were studied. Soil macropore 3D reconstruction was achieved by visual interactive system VolView 3.4 to investigate the connectivity, branchedness and complexity of macropore.[Results] The macropores consisting of root channels, faunal tunnels, shrinkage cracks and inter-aggregate porosity were broadly found in vadose zones of vegetated slope soils. Heterogeneity in distribution of soil macropores was significant, and macroporosity decreased with the increasing soil depth. As clearly demonstrated by 3D reconstruction, macropores were well distributed and connected in the soil cores.[Conclusion] We highlight the importance of CT technique in soil macropore quantification and 3D reconstruction.