[Objective] In high-altitude regions, engineering and construction activities will inevitably damage the fragile alpine meadows, leading to the exposure of the land surface and triggering a series of ecological problems, such as soil erosion. Turf transplantation and replanting are effective methods for protecting and restoring alpine meadows. In practice, to address land occupation issues, the removed turf is often stacked in multiple layers before replanting. However, traditional layering and replanting methods are associated with the disadvantage of low survival rates. Therefore, this study investigated techniques for turf stacking and replanting. [Methods] We performed artificial turf stripping and conducted stacking and replanting experiments with varying turf layer numbers (2 to 5 layers) at the La'u Mountain, Mangkang County, Tibet Autonomous Region. We analyzed turf preservation rates during stacking, turf survival rates after replanting, and plant species composition under varying layer numbers and seasons. We explored the key technical aspects of turf stripping, stacking, and replanting. [Results] The results showed a turf stacking preservation rate of 96.89%, and the preservation rate increased significantly when more than two layers were stacked. The survival rate after turf replanting was 87.96%, and there was no significant difference in survival rate between different stacking layers. Turf stacking led to a reduction in species diversity and a simplification of community structure; after replanting, species diversity gradually recovered to levels similar to the original vegetation. Considering spatial efficiency and turf survival rate, we recommend stacking turf in five layers. [Conclusions] This study optimized turf stripping, stacking, and replanting measures for vegetation restoration, providing a solid scientific foundation and valuable technical knowledge for the protection of alpine meadow resources.