[Objective]To investigate the effects of tartaric acid (TA) as an amendment on the respiratory activity of reclaimed soil, this study aims to reveal the dynamic variation patterns of soil respiration under TA addition and its interactive effects with environmental factors.[Methods]Taking the reclaimed soil from the Panyi Mine reclamation area in northwestern Huainan as the research material, a gradient-controlled experimental design was adopted. Gradients of TA application rate and concentration were systematically set, coupled with dual environmental factor gradients of temperature and soil moisture content. Long-term continuous in-situ monitoring of the respiration rate of reclaimed soil was conducted throughout the experiment.[Results](1) Compared with the control group (TA application rate = 0), TA addition at a rate lower than 675 mg·Kg-1 promoted the respiratory activity of reclaimed soil. In contrast, TA addition at a rate higher than 675 mg·Kg-1 exhibited an inhibitory effect on soil respiration.(2) When soil temperature and moisture content changed, the daily cumulative CO2 emission from reclaimed soil showed a trend of first rapidly increasing and then decreasing during the incubation period after TA addition. The promotion effect of TA on soil respiration followed the order of temperature groups: TA-35℃ > TA-25℃ > TA-15℃, and moisture content groups: TA-25% > TA-15% > TA-35%. At 35℃, the average daily cumulative respiration over 20 days was 11.03 times higher than that of the control group (CK); at 25% soil moisture content, it was 5.14 times higher than that of the CK group.(3) Redundancy analysis (RDA) indicated that TA concentration was the key factor affecting the respiratory activity of reclaimed soil, followed by temperature.[Conclusion]Appropriate addition of TA can significantly increase the CO2 respiration rate of reclaimed soil, drive the dynamic changes of soil nutrient content, optimize the physicochemical and biological properties of reclaimed soil, and thereby contribute to the long-term stable utilization of reclaimed soil.