[Objective] The evolutionary trends and regional differences of agricultural green development level in Gansu Province were analyzed, aiming to provide crucial theoretical support for promoting regional agricultural green development and achieving rural revitalization. [Methods] An evaluation indicator system for agricultural green development level was constructed. The entropy weight method was used to measure the agricultural green development level in Gansu Province and its 14 prefectures and cities. Furthermore, kernel density analysis and Dagum Gini coefficient were employed to thoroughly investigate the dynamic evolution and regional differences of agricultural green development level among the prefectures and cities. [Results] ① From 2014 to 2023, the overall agricultural green development level in Gansu Province showed an upward trend, with an increase of 17.65%. Additionally, significant spatial differences were observed in regional agricultural green development level, generally showing gradient distribution characteristics of Hexi region > Longdong region > Longzhong region. ② The period from 2014 to 2016 was the initial stage of agricultural green development in Gansu Province. From 2017 to 2019, the agricultural green development level increased significantly across the province. From 2020 to 2023, it entered a stage of fluctuating adjustment. ③ From 2014 to 2023, the regional differences in agricultural green development level in Gansu Province exhibited a trend of initial rise followed by fluctuating decline, maintaining within a reasonable range (<0.40) overall. The contribution of inter-regional differences was the largest (with an overall contribution rate of approximately 52.33%), especially between Hexi region and Longzhong region. The disparity between the Hexi region and Longdong region was the primary cause of the imbalance in agricultural green development. [Conclusion] From 2014 to 2023, the agricultural green development level in Gansu Province steadily increased, forming a spatial distribution pattern of ‘Hexi > Longdong > Longzhong’, and went through an evolutionary process of ‘initial development—rapid improvement—fluctuating adjustment’. Currently, the agricultural green development level in the Hexi region is substantially higher than that in the Longzhong and Longdong regions.