A pot experiment was performed with five treatments,i.e.CK(neither urea nor chicken manure was applied),N₁₀₀ (100% of nitrogen was provided by urea),M₁₀N₉₀(10% and 90% of nitrogen were provided by chicken manure and urea,respectively),M₃₀N₇₀(30%and 70%of nitrogen were provided by chicken manure and urea,respectively) and M₃₀N₇₀ (50%and 50%of nitrogen were provided by chicken manure and urea,respectively).The experiment was to determine the effect of chicken manure co-applied with inorganic fertilizer on the photosynthesis,chlorophyll content and root activity and construction,as well as the growth and nutrient uptake of one-year-old poplar seedlings.Results showed that the photosvnthetic(P_n),transpiration rate(T_r),stomatal rate(G_s)and intercellular concentration(C_i)of poplar seedlings leaves in M₃₀N₇₀ treatment were significantly increased as compared with that in N₁₀₀ treatment.M₃₀N₇₀ treatment also resulted in the higher chlorophyll content and root activity,with increases of 45.80% and 36.39% as compared with the N₁₀₀ treatment,respectively.Chicken manure co-applied with inorganic fertilizer had beneficial effects on the root growth of poplar seedlings,especially the weight and the ratio of fine root.In addition,compared with the N₁₀₀ treatment,the M₃₀N₇₀ treatment significantly increased the root，shoot and dry leaf weight accumulation,as well as the N,P and K contents.Consequently,the N,P,and K uptakes increased by 44.99%,65.11%and 65.04%,respectively.However,in M₁₀N₉₀ and M₅₀N₅₀ treatments,less effect on photosynthesis characteristics and the nutrient uptake of one-year-old poplar seedlings was observed than that in M₃₀N₇₀ treatment.Chicken manure co-applied with inorganic fertilizer,especially the M₃₀N₇₀ treatment,achieved the best effects on the growth and nutrients uptake of poplar seedlings.