[Objective] The response of the ecosystem service system to vegetation change in the Qinghai-Tibet Plateau conservation area from 2000 to 2010, and to further clarify the relationship between ecosystem services and remote sensing vegetation index were studied in order to provide a scientific reference for regional ecosystem service assessment and ecological protection.[Methods] This study focuses on various types of protected areas on the Tibetan Plateau and uses MODIS (moderate-resolution imaging spectroradiometer) EVI (enhanced vegetation index) products for vegetation change mapping. The ecosystem services such as carbon fixation, soil conservation, water conservation and habitat protection are used to determine the consistency of ecosystem services and EVI changes in the Qinghai-Tibetan Plateau conservation area from 2000 to 2010.[Results] The EVI of the Qinghai-Tibetan Plateau was dominated by non-significant changes from 2000 to 2010, and the non-significant increased samples were greater than the non-significantly decreased samples in both the grid and the protected areas, indicating that the vegetation on the Qinghai-Tibet Plateau is characterized by overall improvement and local degradation.[Conclusion] The ecological system services in the Qinghai-Tibet Plateau conservation area were mainly non-significant changes from 2000 to 2010, but the non-significant reduction samples of ecosystem services was significantly more than the non-significant increased samples, which was inconsistent with the change trend of EVI.