[Objective] The objective of the study is to research a new biological phosphorus fertilizer in order to improve crop yield in alkaline soil in the Yellow River Delta. [Methods] The cultivating method used in this study was inorganic phosphorus liquid culture medium. Fungi Aspergillus tubingensis(CT₁) was screened from the rhizosphere soil in the garden of Yellow River delta. We researched the phosphate-solubilizing ability of fungi. [Results] The phosphate-solubilizing ability of fungi CT₁ was reduced with the increasing of fermented liquid salt concentration. When the concentration of NaCl was between 0.03% and 6%, the concentration of available phosphorus was maintained between 523.5 and 338.5 mg/L. There was an obvious correlation between the available phosphorus concentration and fermented liquid pH vale. CT₁ could grow normally under four different kinds of carbon sources, and the glucose was the best culture medium. CT₁ could grow normally under four different kinds of nitrogen source, and (NH₄)₂ SO₄ was the best medium. Compared with uninoculated wheat, fungi CT₁ inoculated wheat, after 15 days of wheat cultivation, the length of the stem increased by 16.24%, the fresh weight of the stem increased by 12.35%, and the root length increased by 21.6%. [Conclusion] Phosphate solubilizing bacteria CT₁ could promote crop yield of saline-alkali soil, and can be used as a new phosphate-solubilizing fertilizer.