[Objective] In order to provide reference for similar water transfer projects, the configuration pattern and effects of soil and water conservation measures in inverted siphon project were discussed.[Methods] According to the field investigation and documentary data of the inverted siphon project in Zhengzhou City section of the middle route of the South to North Water Transfer Project in He'nan Province, the layout of soil and water conservation measures in three main construction periods was determined, the configuration pattern of soil and water conservation measures in the inverted siphon project was constructed, and the effect after implementation was analyzed and evaluated according to the field survey data and photos.[Results] Taking the inverted siphon project of Zhengzhou City section as an example, soil and water conservation measures were determined based on the project layout, natural conditions, construction technology and the characteristics of soil erosion in different periods:① Surface soil should be peeled off before construction, together with temporary soil heaping, centralized stacking, blocking, covering or sowing with grass seeds; ② Temporary iron fences, interception and drainage ditches should be set up in the construction area; ③ Cut off drainage and anti-seepage furrow should be installed on the excavated slope and the tarpaulin should be timely covered; ④ Ecological landscape areas should be set up from the dam to the protection area; ⑤ Drainage ditches, frame planting grass slope protection and triangular small green space should be set up in the gradual transition sections on both sides of the inlet and outlet; ⑥ Landscaping, drainage and sand sinking measures should be set up in the operation management area, and permeable bricks should be laid in parking lots; ⑦ A leisure ecological park built on the top of the siphon become an allocation model of soil and water conservation measures in the inverted siphon project, which has achieved good results after implementation.[Conclusion] The inverted siphon project is not only an important part of water diversion project, but also a kind of cross building with the largest number in the first phase of the middle route of the South to North Water Transfer Project. The reasonable layout of engineering, plant and temporary measures according to the location and the construction of soil and water conservation measure system will certainly achieve better results on soil and water conservation and greatly improve the ecological environment, and provide a reference for similar projects to compile scientific, economic, safe and feasible scheme.