Abstract:[Objective] The land use change in Hami City, Xinjiang Uygur Autonomous Region, over the past 20 years was studied to provide strong support for land use planning and ecological environment protection.[Methods] Based on the GlobeLand 30 datasets in 2000, 2010, and 2020, the spatial-temporal change characteristics of land use in Hami City from 2000 to 2020 were analyzed using a transfer matrix, a slope distribution index, a standard deviation ellipse, and the land use and use center-of-gravity migration.[Results] ① The land use change was obvious in Hami City from 2000 to 2020. The proportion of land use types in 2020 was in the order of unused land > grassland > cultivated land > forest > construction land > water area. Although Hami occupied a large area, the usable oasis area was small. In 2020, the grassland area was 10 226.39 km2, and the water area had the smallest proportion, with an area of 295.17 km2. In terms of land use type change from 2000 to 2020, grassland had the largest reduction, and the loss area was 671.48 km2. It was mainly converted into unused land, forest, and a small amount of cultivated land. The increase in construction land was the largest, with an area of 405.60 km2. From 2000 to 2020, the area of forest and cultivated land showed an increasing trend, while the unused land and water areas showed a decreasing trend. The reduction of water area was very small, and the area remained stable. ② From 2000 to 2020, grassland was evenly distributed on the five slope gradients. The cultivated and construction land were distributed in grade Ⅰ and grade Ⅱ slope gradients, and cultivated land was dominant in grade Ⅰ slope gradient. The area of forest was the largest on grade Ⅰ gradient. The distribution area of unused land decreased with the increase in the slope gradient. ③ Compared with 2000-2010, the land use change in 2010-2020 had a more obvious directionality and wider range, and the center of gravity shifted to the northwest.[Conclusion] From 2000 to 2020, the land use change of Hami City had obvious space-time characteristics, and the influence of the slope on the distribution of various types is significant.