[Objective] The changes of land use and carbon storage under different scenarios were analyzed to provide the scientific bases were provided for optimizing of land use pattern and formulating protection policies in Hainan Tropical Rainforest National Park. [Methods] Based on the land use data of Hainan Tropical Rainforest National Park in 2001, 2011 and 2021, and the PLUS and InVEST models, the changes of land use and carbon storage in 2001—2021 and 2030 under different scenarios were analyzed and predicted, respectively. [Results] ① From 2001 to 2021, the cultivated lands, grassland and water decreased continually, while the forest land and construction land gradually expanded in Hainan Tropical Rainforest National Park. And the forest land increased by 561.19 km² in 20 years. The predicting results of land use changes under natural development scenario and farmland protection scenario in 2030 were similar but were much different with the results of ecological protection scenario. ② In 2001, 2011, and 2021, the carbon storage of Hainan Tropical Rainforest National Park was 8.72×10⁷ t，9.22×10⁷ t，and 9.13×10⁷ t, respectively. In 2030, the carbon storage of Hainan Tropical Rainforest National Park would be 9.04×10⁷ t under natural development scenario, 9.12×10⁷ t under farmland protection scenario, 9.13×10⁷ t under ecological protection scenario. These results indicating that the ecological protection measures could improve the ability of carbon storage in Hainan Tropical Rainforest National Park. [Conclusion] The change of carbon storage was highly consistent with the change of land use, and land use change could affect the pattern of carbon storage in Hainan Tropical Rainforest National Park.