[Objective] To scientifically identify the agglomeration characteristics and evolution trend of land use conflicts, so as to provide important reference for promoting the rational layout of territorial space and promoting regional sustainable development. [Methods] The spatial and temporal evolution trend of land use conflict in Wanjiang Urban Belt from 1990 to 2020 was quantitatively analyzed by constructing a land use conflict measurement model based on landscape index, and the distance weight matrix was determined by setting the optimal spatial threshold, so as to explore the agglomeration effect of regional conflict more accurately.[Results] (1) Great changes have taken place in the land use structure of Wanjiang Urban Belt in recent 30 years. The area of cultivated land and forest land has been continuously reduced, and the scale of construction land has increased significantly. (2) The distribution pattern of regional land use conflicts is "high in the north and low in the south", and the area of weak and medium spatial conflicts occupies the main position; The serious conflict areas are concentrated in Hefei City, Wuhu City, Lu 'an Jin 'an District and the coastal areas on both sides of the Yangtze River system; (3) The land use conflict index changed significantly in urban areas during the study period, especially in the east of Hefei, the north of Wuhu and the southeast of Chuzhou. Under the influence of urbanization development, the center of gravity of conflict gradually shifted to the south, and the serious conflict zone shifted to the northwest to the southeast. (4) 5km was selected as the optimal distance analysis threshold. The high-high aggregation conflict zone was distributed in sections centered on Hefei City, while the low-low aggregation conflict zone did not have agglomeration phenomenon. [Conclusion] The land use conflict in Wanjiang Urban Belt has potential to intensify, and the main conflict areas show a shifting trend. In the future, it is necessary to strengthen the rational distribution of regional land use and pay attention to the balance and stability between urbanization development and ecological protection. The results of this study can provide practical basis and reference for conflict management in Jianghuai region and other small and medium-sized urban belt.