［Objective］ This study aims to identify the spatial conflict patterns of land use within the Southern Anhui International Cultural Tourism Demonstration Zone and to investigate their correlation with land use change， in order to provide a scientific basis for regional coordinated and sustainable development. ［Methods］ A land use conflict measurement model based on landscape pattern indices was constructed， and quantitative analyses to characterize land use change， land use conflict， and land use intensity from 2000 to 2023 were conducted combining the geographic detector method to examine the driving mechanisms. ［Results］ ① From 2000 to 2023， land use within the zone showed a trend of cultivated land and forest land decreasing， while construction land expanded. Land conversion hotspots concentrated in the northern riverside plains and southern intermountain basins. Forest land maintained its ecological substrate function through bidirectional conversions. ② The level of land use conflict exhibited a spatial pattern of higher values in the north and lower values in the south， but the overall trend decelerated. Land use intensity decreased at the same time， resulting in a differentiated pattern characterized by low-intensity strips in mountainous areas and high-intensity nodes in plains. ③ Spatial synergy between land use conflict and land use intensity significantly strengthened during the study period. The overall Moran’s I index increased continuously， and high-value agglomeration areas overlapped with hotspots of construction land expansion. ④ Slope emerged as the dominant natural environmental factor constraining the conflict pattern. The interaction level between land use intensity and GDP was comparable to that between land use intensity and natural environmental factors， which revealed a trend where economic development intentions challenge natural constraints. This dual-driver effect was supported by the finding that 56.6% of lost cultivated land was converted for urban construction. ［Conclusion］ While the overall level of land use conflict decreased within the Southern Anhui International Cultural Tourism Demonstration Zone， spatial differentiation intensified because of both natural and economic factors. Strict control of development in the northern plains through territorial spatial use regulation remains necessary. At the same time， coordination of ecological protection and intensive land use across the entire region is required to drive high-quality development of the International Cultural Tourism Demonstration Zone.