[Objective] The impact of different human disturbances on the health of a soil-grassland system was evaluated quantitatively to provide a scientific basis for grassland ecological restoration, formulation of management measures, and sustainable utilization. [Methods] Using the grassland of Xilamuren in Inner Mongolia as the research object, we compared and analyzed the physicochemical properties and vegetation characteristics of the grassland soil under the four types of disturbance methods, namely enclosure, grazing, reclamation and tourism, and used grey correlation analysis and the fuzzy comprehensive evaluation method to assess the system health status. [Results] ① The percentage contents of gravel and sand were highest in the tourist area (14.98% and 67.79%, respectively) and lowest in the enclosure area (9.02% and 55.38%, respectively). The highest percentages of clay (15.45%) and silt (20.13%) were found in the enclosure zones, whereas the lowest values (7.21% and 10.02 %, respectively) were found in the tourism zones. The highest soil bulk density (1.61 g/cm3) was also found in tourism zones. No significant differences were detected in the soil moisture and saturated water-holding capacity within the soil layers of the sampling sites. ② The highest soil organic matter (12.98 g/kg) and total nitrogen (0.73 g/kg) were observed in enclosure zones, whereas the lowest (8.23 g/kg and 0.46 g/kg) values were found in tourism zones. The total phosphorus and potassium levels among the different human disturbances were ranked as follows: reclamation > enclosure > grazing > tourism. Furthermore, 9.08 mg/kg more available phosphorus in reclaimed areas was higher than in tourism zones, whereas the content of available potassium (268 mg/kg) in the enclosure zones was significantly higher than that in other sites. ③ Total plant biomass of sampling site was ranked as enclosure (342.67 g/m2) > reclamation (155.29 g/m2 > grazing (112.93 g/m2) > tourism (43.51 g/m2). ④ Soil quality and grassland health were ranked as follows: enclosure > reclamation > grazing > tourism. [Conclusion] Significant differences in the soil physicochemical properties and plant biomass were observed under different human disturbance. Enclosure zones demonstrated the best soil quality and grassland health, whereas tourism zones showed the worst conditions. Both reclamation and grazing negatively affected soil quality and grassland health.