Concentration and chemical speciation of heavy metals(Zn, Cu, Pb, Ni) in soil and tea collected from tea garden in South Anhui Province were analyzed by Tessier's sequential extraction method. Bioavailability of heavy metals were assessed by the ratio of active state to total amount. Results showed that concentrations of Zn, Cu, Pb and Ni in the tea garden soils were lower than that of the national secondary standard. Cu, Pb, Ni concentrations exceed soil background value of South Anhui Province, which indicated there were obvious enrichment phenomenon. Zn, Cu, Ni mainly existed as residual speciation, accounted for 72.55%, 90.00%, 81.79% of the total respectively; Pb existed in iron and manganese oxides, accounted for 70.09%; The active speciation(bioavailability) ratio of Zn, Cu, Pb, Ni were 5.04%, 1.51%, 0.97%, 0.23%, respectively. Active speciation of heavy metal in soil showed significantly positive correlation with the concentration of heavy metal in tea. Concentration of heavy metals is in the range of limited value and is safe in tea of South Anhui Province.