The indicators system of water resources pressure were was developed according to water resources quantity pressure, water resources economic pressure and water environmental pressure. Then, this indicator system was used for calculating water resources pressure index in Heihe River from 2000 to 2010. Results showed that the water resource pressure index in Heihe River was high, but overall, it showed a decreasing tendency. The index of water pressure had fallen from 0.58 in 2000 to 0.28 in 2010. This was attributed to the integrated river basin management including the water-saving society construction. However, It is still necessary to reduce water use in agriculture and increase ecological water use, to reduce the pressure and promote sustainable utilization of Heihe River basin water resources.