[Objective] Soil bio-characteristics of Lonicerae hypoglauca with different planting years was studied in karst mountainous area to provide the basic data for the improvement of L. hypoglauca cultivation in Guizhou Province.[Methods] We used the field observation and laboratory analysis in the study. Soil samples from the root zone of L. hypoglauca were collected to investigate soil bio-characteristics with different planting years in Suiyang County of Guizhou Province.[Results] The amount of microorganism and bacteria(B) in the soil increased in the initial planting years and then decreased gradually, while the amount of actinomycetes(S) and fungi(F) in the soil increased continually with the addition of the planting years. Similarly, the content of C, N and P of soil microorganisms showed an increasing trend at the initial planting years and then decreased with the addition of planting years. The activity of both catalase and urease increased initially and then decreased. In contrast, soil phosphatase showed a decreasing trend initially and then increased with the addition of planting years. The B/F ratio of soil increased initially and then decreased. The number of actinomycetes/fungi(S/F) increased with the planting year. The Shannon-Wiener index of soil microorganisms increased initially and then decreased, while both of the Simpson index and Shannon evenness index decreased with the addition of planting years.[Conclusion] Soil fertility declines and the incidence of virus in root zone increases with the addition of planting year.