[Objective] The aim of this study is to reveal the spatial and temporal variation of regional ecological risk in rapid urbanization areas(Qingpu District of Shanghai City), in order to provide technical support for regional ecological planning and construction.[Methods] With the employment of GIS technology, the regional integrated ecological risk assessment model was constructed based on landscape pattern index. Spatial distribution of ecological risk in the study area was analyzed by spatial autocorrelation.[Results] The land use in the study area changed greatly from 2000 to 2013. The area of forest and urban increased gradually while the area of farmland decreased, wetland remained stable. The spatial variation of ecological risk in the study area was significant. The ecological risk of the area in the eastern was higher than that of the area located in the west, and the expansion speed of ecological risk in the east was higher than that in the west. Urbanization had a significantly impact on ecological risk.[Conclusion] Regional ecological risk has significant spatial agglomeration characteristics, and its ecological risk value represents a significant positive correlation. These changes are closely related to ecosystem change and human activities.