[Objective] The spatial-temporal variation of the eco-environmental quality in He'nan Province was analyzed to provide scientific bases for the monitoring, conservation and management of eco-environmental.[Methods] This paper evaluated eco-environment quality by eco-environmental quality index. The eco-environmental quality index was calculated by five indexes, which included the biological richness, vegetation coverage, water network denseness, land stress and pollution load. These indexes were calculated based on the grid-based collected multi-source and multi-temporal data, and technical criterion for eco-environmental status evaluation was also considered.[Results] (1) The main type of eco-environmental quality was "general" in He'nan Province, and it accounted for nearly 80% coverage of the whole areas, other types accounted for relatively less. The topography, land use types and regional development strategy had profound effects on the distribution of eco-environmental equality. (2) The eco-environment quality has showed a trend of reduction with small annual variation in recent years. Chronologically, it showed a unimodal distribution, showing no significant changes in the highest years, and the significant changes in the early and laters sides years. (3) The changes of eco-environmental quality were closely related to the regional development, which had showed a deterioration trend in recent 13 years. Influenced by the expansion of urban construction land, the degeneration regions had shifted from the midwestern to the southeast of He'nan Province.[Conclusion] The evaluation of eco-environmental quality based on gridding could reflect the spatial distribution characteristics and the influencing factors of regional ecological environment more distinctly. In order to improve the quality of ecological environment, regional development strategy must be coordinated with the ecological civilization construction.