[Objective] The effects of land use on humus (humic acid, fulvic acid and humin) and four kinds of important soil enzymes in different yellow soil horizon under soil erosion condition were studied in order to provide some scientific basis for maintaining soil fertility.[Methods] Soil samples were collected in different soil horizon (0-10 cm, 10-20 cm and 20-30 cm) at each sample point by zigzag method. Then, soil samples of each layer were mixed evenly in situ and removing of redundant sample was performed by quartering in three times and the corresponding indicators were determined.[Results] (1) Scrub woodland and garden plot had better effect in maintaining soil fertility. However, cultivated land was extremely susceptible to erosion. Soil fertility was directly related with the content of humin of yellow soil. (2) Soil erosion may also affect humic acid content through the loss of water and nitrogen. Fulvic acid of soil in forest land, garden plot and cultivated land lost more easily. However, the grassland had only impact on maintain soil surface fertility. (3) Tillage had significantly adverse effect for activity of urease, alkaline phosphatase and catalase. Meanwhile, soil erosion also evidently affects soil enzymes through the change of soil pH value. (4) Correlation analysis showed that the content of humus component had close contact with the activities of the four kinds of soil enzymes and influenced each other.[Conclusion] The land use types of yellow soil have significantly different effects on humus and soil enzyme activity under erosion.