盐碱胁迫对柳树抗氧化酶的影响
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中央级公益性行业科研院所基本科研业务费“柳树抗复杂盐胁迫机理研究”(CAFYBB2016MA011);江苏省科技支撑计划项目(BE2016328)


Effects of Salt and Alkali Stresses on Antioxidases of Willow
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    摘要:

    [目的] 探讨柳树抗氧化系统对盐胁迫和碱胁迫响应的差异机制,以期为今后耐盐碱柳树选育工作提供科学依据。[方法] 以盐柳1号为试验材料,分别以中性盐NaCl和NaSO4、碱性盐NaHCO3和Na2CO3混合模拟盐、碱胁迫(两者摩尔比均为9:1),共设计了5个梯度处理(0~200 mmol/L),共胁迫14 d来研究盐与碱胁迫下柳树幼苗相关生理表现。[结果] 在碱胁迫情况下随着盐浓度升高,柳树叶片的SOD,POD,CAT这3种酶活性呈先升后降变化趋势,其中SOD在盐浓度为100 mmol/L时达到最大为对照的1.48倍,POD和CAT在盐浓度为150 mmol/L达到最大,分别为对照的4.26和5.24倍。中性盐胁迫下SOD和POD活性一直保持增大,在盐浓度为200 mmol/L时达到最大值分别对照的1.33和3.26倍,在盐浓度为150 mmol/L时CAT活性达到最大值为对照的2.79倍。碱胁迫下柳树叶片中丙二醛(MDA)含量明显高于中性盐胁迫下,当盐浓度为150 mmol/L时为同浓度中性盐胁迫下的2.23倍。[结论] 从氧化胁迫角度分析,碱胁迫和盐胁迫是两种不同性质的胁迫,碱胁迫对柳树抗氧化系统造成的危害更大。

    Abstract:

    [Objective] To illustrate the comparative effects of salt and alkali stresses on antioxidases of willow, and to provide scientific basis for willow breeding.[Methods] A willow cultivar Salix psammophila (Yanliu yihao) was used in this study. The stress conditions were simulated by five levels of salinities and alkalities with 14 days duration. The salinities were simulated by two neutral salts (NaCl and NaSO4) and alkalities were simulated by two alkaline salts (NaHCO3 and Na2CO3). The two neutral and alkaline salts were both mixed in the molar ratio of 9:1. The concentrations ranged from 0 to 200 mmol/L both for salt and alkali stress.[Results] Under alkali stress, the activities of superoxide dismutase (SOD), peroxidase(POD) and CAT all presented a tendency of increasing at low concentration stress and then decreasing at high stress. The peak of SOD activity emerged when the willow was treated with 100 mmol/L, which was 1.48 times of the control's; the peak of POD and CAT activities emerged when it was treated with 150 mmol/L, which were 4.26 and 2.79 times of the control's values. Under alkali stress, the activities of SOD,POD and CAT all presented an increasing tendency. The peaks of SOD,POD and CAT activities all emerged at highest contrcentration of 200 mmol/L, they were 1.33,3.26 and 2.29 times of the control's corresponding values. The content of MDA of willow leaf was higher in alkali treatment than that in salt treatment. When the concentrations of neutral and alkaline salts were at 150 mmol/L, the difference of MDA content was biggest.[Conclusion] The alkali stress is different from salt stress, the alkali has stronger injury to willow than the salt stress has.

    参考文献
    [1] 史小丽,叶茂,王晓峰,等.NaCl胁迫对塔里木河下游罗布麻抗性酶的影响[J].干旱区资源与环境,2011,25(5):209-212.
    [2] 雷金银,班乃荣,张永宏.柽柳对盐碱土养分与与盐分的影响及其区化特征[J].水土保持通报,2011,31(2):73-76.
    [3] 李彬,王志春,孙志高,等.中国盐碱地资源与可持续利用研究[J].干旱地区农业研究,2005,23(2):154-158.
    [4] 武德,曹帮华,刘欣玲.盐碱胁迫对刺槐和绒毛白蜡叶片叶绿素含量的影响[J].西南林学院学报,2007,22(3):51-54.
    [5] Lv Bingsheng, Li Xiaowei, Ma Hongyuan, et al. Differences in growth and physiology of rice in response to different saline-alkaline stress factors[J]. Agronomy Journal, 2013,105(4):1119-1128.
    [6] 张建,李敏,李玉娟,等.盐胁迫下3个柳树新品系生理变化[J].江苏农业科学,2012,40(8):197-199.
    [7] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2006:119-124.
    [8] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2001.
    [9] 代莉慧,蔡禄,周耀龙,等.NaCl和Na2CO3胁迫对内蒙古河套灌区盐地碱蓬种子萌芽生理指标的影响[J],种子,2013,32(7):14-17.
    [10] 刘金文,佟丹丹,关利娅.NaCl胁迫对不同苜蓿品种超氧化物歧化酶同工酶的影响[J].黑龙江八一农垦大学学报,2006,18(6):4-7.
    [11] 周建,刘弘,尤杨等.碱胁迫对紫荆幼苗生长与光合作用的影响[J].东北林业大学学报,2008,30(6),13-15.
    [12] 颜志明,魏跃,贾思振,等.盐胁迫对草莓抗氧化系统和离子吸收的影响[J].北方园艺,2013(9):1-4.
    [13] 周瑞莲,王海鸥,赵哈林.不同类型沙地植物保护酶系统对干旱、高温胁迫的响应[J].中国沙漠,1999,19(1):49-54.
    [14] Bowler C, Montagu M, Inze D. Superoxide dismutase and stress tolerance[J]. Annual Review of Plant Biology, 1992,43(1):83-116.
    [15] van Camp W, Capiau K, van Montagu M, et al, Enhancement of oxidative stress tlerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts[J]. Plant Physiology,1996,112(4):1703-1714.
    [16] Meloni D A, Oliva M A, Martinez C A, et al. Photosynthesis and activity of superoxide dismutase, peroxide and glutathione redutase in cotton under salt stress[J]. Environmental and Experimental Botany,2003,49(1):69-76.
    [17] 张继澍.植物生理学[M].北京:高等教育出版社,2006:431.
    [18] 袁琳,克热木·伊力.NaCl胁迫对阿月浑子实生苗活性氧代谢与细胞膜稳定性的影响[J].植物生态学报,2005,29(6),985-996.
    [19] 李会云,郭修武.盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J].果树学报,2008,25(2),240-243.
    [20] 马丽清,韩振海,周二峰,等.盐胁迫对珠美海棠和山定子膜保护酶系统的影响[J].果树学报,2006,23(4),495-499.
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刘铎,丛日春,高卫东,党宏忠,李庆梅,刘德玺,杨庆山.盐碱胁迫对柳树抗氧化酶的影响[J].水土保持通报,2017,37(5):53-57

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  • 收稿日期:2014-08-08
  • 最后修改日期:2017-04-25
  • 在线发布日期: 2017-11-14