[Objective] This paper illustrated the influencing factors of water resource security and its evolution trend aimed to put forward some suggestions about the water resource security in Karst area.[Methods] Based on the data of Guizhou Province in the past ten years, this paper established a BP network model and applied the mean impact value(MIV) algorithm method to analyze the influencing factors of the water resource security in Karst area.[Results] The groundwater supply ratio, the industrial water use proportion, water use efficiency, per capital grain yield and water yield modulus were the obstacles to the development of water resources system. The influencing degree of water yield modulus was decreasing year by year, while the other four factors showed increasing trends. Comprehensive utilization rate of industrial solid waste, the attainment rate of the industrial waste water, the ratio of moderate rocky desertification area, water requirement per-unit GDP and exploitation degree of groundwater were the driving factors. In chronological order, the influence of water requirement of per-unit GDP and exploitation degree of groundwater were stable, while the ratio of moderate rocky desertification area was more and more pronounced. The impact of the attainment rate of the industrial waste water decreased year by year, while comprehensive utilization rate of industrial solid waste fluctuated greatly.[Conclusion] The MIV-BP model is feasible in studying influencing factors of water resource security in karst area.