[Objective] The impacts of land use change on the ecosystem service value of various natural process were studied, in order to provide scientific basis for rational utilization of land resources and ecological security protection of the basin.[Methods] Based on the change of land use transformation, this paper analyzed the change of land use and the change of ecosystem service value in Yongjiang River basin under the influence of human activities from 1980 to 2015.[Results] ① The decrease of cultivated land and the increase of construction land were the main characteristics of land use change in Yongjiang River basin, whose degree of land use is rising. The land use types showed complicated mutual transformation, and the largest transfer area was from the conversion of cultivated land to construction land. ② During the past 35 years, the dynamic degree of land comprehensive utilization was 0.42%. The single dynamic degree of construction land use was the largest, and the reduction of cultivated land area was the greatest. ③ In terms of time, the total value of ecological services in the Yongjiang River basin increased first and then decreased, and increased continuously before 1995, but decreased continuously afterwards. ④ At the spatial level, the total value of ecological services showed a decreasing trend from southwest to northeast.[Conclusion] The transformation of land use in Yongjiang River basin was intense, and the rapid change of land use has aggravated the degradation of ecosystem function.