[Objective] The distribution of production, living and ecological space in Duyun City, Guizhou Province, was studied to provide theoretical guidance for the sustainable development, county-level land planning, and conservation of environment in karst areas.[Methods] Lorenz curves, Gini coefficients, and transition models were used for the comprehensive study of the production, living and ecological space in Duyun City from 2009 to 2018.[Results] ① In 2018, ecological space constituted 69.19% of the total area, followed by production space, which accounted for 26.87% of the total, and living space accounting for the smallest percentage, which accounted for 3.94%. ② From 2009 to 2018, the areas of production space and the ecological space showed a downward trend, whereas that of living space showed an upward trend. In terms of secondary space transfer, the increased areas of urban and rural living space came from agricultural production space and forest ecological space, and urban development occupied a part of forest land resources and agricultural resources. ③ In terms of Lorenz curves and Gini coefficients from 2008 to 2019, ecological space and production space were evenly distributed. Whereas, compared with the other two types, the curve of living space diverted farther away from the line of perfect equality, showing a concave curve. Gini coefficients from 2009 to 2018 were between 0.3 and 0.4, and the distribution of living space was basically reasonable. ④ From the perspective of location entropy, areas with an advantage of ecological space were mainly concentrated in the western part of the study area, areas with an advantage of living space were mainly concentrated in the central part of the study area, and areas with an advantage of production space were mainly concentrated in the southern and eastern part of the study area.[Conclusion] The three types of space were appropriately distributed in Duyun City, with ecological space as the dominant type. Future urban construction should be premised on the preservation of ecological space, with planning for urban development while limiting the use of ecological land.