[Objective] The impacts of the process of land use evolution on ecosystem service value in Jilin Province were analyzed and predicted in order to provide theoretical basis for the formulation of ecological environment protection and improvement policies and strategies in Jilin Province.[Methods] Based on the land use data of Jilin Province in 2000, 2010 and 2017, the CA-Markov model was used to simulate and predict the land use pattern in 2024, and InVEST model was used to analyze the change of ecosystem service value and the spatial-temporal evolution characteristics of habitat quality in Jilin Province from 2000 to 2024.[Results] ① From 2000 to 2024, the area of cultivated land, grassland and construction land increased continuously, while the area of woodland, water area and unused land decreased. ② From 2000 to 2024, the total value of ecosystem services showed a downward trend, among which forest ecosystem services value was the largest, and hydrological and climate regulation were the main ecological service functions. ③ The spatial distribution of habitat quality in Jilin Province was low in the central and western regions and high in the east. The quality of the habitat is deteriorating continuously, and the degradation is serious around the city and along the main river basins.[Conclusion] The habitat quality in the central and western regions of Jilin Province has declined seriously, thus, more attention should be paid on the protection and rational utilization of city surrounding zones and water systems.