[Objective] The temporal and spatial evolution characteristics of land use and ecological service value (ESV) in the urban agglomeration on the northern slope of Tianshan Mountains were studied in order to provide theoretical guidance for the improvement of ecological service functions and the construction of ecological landscape in this area.[Methods] Based on the land use data of the urban agglomeration on the northern slope of the Tianshan Mountains in 2000, 2010, and 2018, the characteristics of the land use dynamics and the ecological service value of the urban agglomeration on the northern slope of the Tianshan Mountains were analyzed using remote sensing and spatial statistical analysis methods. The landscape index model was used to explore the landscape pattern changes of the urban agglomeration on the northern slope of Tianshan Mountain.[Results] ① The main types of land used in the urban agglomeration on the northern slope of Tianshan Mountains were unused land and grassland. The dynamic change of land use for construction land was the largest. The land use transfer mainly occurred from grassland to cultivated land, cultivated land and unused land. And the transfer from grass land to cultivated land and construction land was the most prominent. ② The total amount of ESV in the study area in the past 20 years decreased from 141.176 billion yuan to 140.017 billion yuan, with a total loss of 1.059 billion yuan. The overall trend was declining, and grassland had the most ESV loss. ③ The ESV of the study area presented a spatial pattern of high in the northwest and middle, and low in the north and southeast. the ESV landscape pattern of the study area changed significantly from 2000 to 2018, and the landscape pattern of the low, medium and high ESV density areas was characterized by fragmentation and heterogeneity in the early stage of the study, and the homogeneity of agglomeration in the later stage.[Conclusion] The ecological environment of the urban agglomeration on the northern slope of the Tianshan Mountains is deteriorating. For different grades of ESV areas, different measures need to be taken to improve the ecological service value of the study area, reduce the damage to the ecological environment caused by the development of the urban agglomeration, and promote the construction of ecological landscape.