[Objective] The ecological corridor in Etuoke Banner, Inner Mongolia was constructed to improve the quality and stability of the ecosystem, and provide the path of biological migration, promote species exchange between different habitats, so as to provide a scientific basis for the implementation of ecological corridor construction project and the protection of biodiversity in Etuoke Banner.[Methods] Taking Etuoke Banner as the research area, the ecological source was determined by using the comprehensive results of ecological sensitivity evaluation and ecosystem service value analysis, and the minimum cumulative resistance model (MCR) and minimum cost path were used to construct the ecological corridor.[Results] ① The extreme and high sensitive ecological area of Etuoke Banner, mainly distributed in the border area of Mengxi Town and Qipanjing Town in the northwest, accounted for 41.31% of whole banner. ② The overall status of ecosystem service value was in poor condition, most of the areas were in the middle and low value areas, accounted for 95.37% of whole banner. ③ The ecological corridor system was constructed, and the ecological corridor and ecological source area roughly formed a ring in the closed area, among them the total length of important corridors was 721 km, and that of general corridors was 803 km.[Conclusion] On the whole, the ecological source areas of Etuoke Banner are mainly scattered on both sides of the east and west. Bulong Gacha and Chahannoer Gacha play a bridge role in the exchange of material and energy between the east and the west. In order to ensure the overall connectivity of the study area, it is particularly important to strengthen the protection of these sources.