Abstract:[Objective] The effects of different soil substrates on the yield and nutritional components of Morchella fruiting bodies were studied in order to provide the practical cultivation techniques of Morchella in the mountainous area of Southern Ningxia Hui Autonomous Region. [Methods] Three soil treatments were used to cultivate Morchella and to determine effects on fruit body yield: ① raw soil and top soil (control group), ② raw soil + sheep manure (ratio 3∶1), and ③ raw soil + shiitake mushroom residue (ratio 3∶1). Agronomic traits and nutrient content were determined. [Results] The mushroom residue treatment group had the highest fruiting density and the highest yield (2 500 kg/hm2). There were significant advantages in stipe diameter, stipe length, cap diameter, and single mushroom weight with this treatment. The water content (18.56 g/100 g) and crude fiber content (35.65 g/100 g) of the fruit body was highest for the mushroom residue treatment. The contents of crude polysaccharides and crude fat were lower for this treatment, and the contents of essential amino acids (3.46%) and of flavored amino acids (2.76%) were highest. [Conclusion] The addition of lentinus edodes chaff to the cultivation of Morchella was beneficial for increasing the yield and nutritional value of Morchella fruiting bodies.