[Objective] The applicability of the InVEST model in the evaluation of the importance of water conservation functions at the city and county level was assesssed, and the spatiotemporal characteristics and regional differences of water conservation services in Guangzhou City, Guangdong Province was analyzed, in order to provide a theoretical basis for regional socio-economic development and the formulation of ecological environmental protection and restoration policies. [Methods] Using comparative analysism, the accuracy of InVEST water conservation evaluation model and related guidelines (Technical Guide for Carrying Capacity of Resources and Environment and Suitability Evaluation of Land Spatial Development) recommended model in regional water conservation services was evaluated. And GIS spatial analysis method was used to analyze the spatial and temporal analysis of water conservation service in Guangzhou City from 2013 to 2017. [Results] ① The evaluation results of the water conservation evaluation model based on InVEST were highly accurate, and the evaluation results of the importance of water conservation functions also met the requirements. ② The total amount of water conservation in Guangzhou City increased first and then decreased, and maintained a certain growth trend. The high values were concentrated in the water area and the forested area in the northeast, while the low values were concentrated in the built-up area of the city center. ③ The overall water conservation function had been improved, but differences existed among different administrative divisions and land types. The administrative regions of Haizhu District, Panyu District and Liwan District had increased greatly, the cultivated land and construction land had been improved, forest land had remained unchanged basically, while grassland had been weakened. ④ The proportion of area of water conservation function importance zone was similar, but the proportion of different land types within each zone was different. [Conclusion] ① Compared with the guide model, the water conservation assessment model based on InVEST is more accurate and has a good application prospect in the field of planning. ② The spatial differentiation of water conservation in Guangzhou City is obvious, and the overall water conservation function has been improved from 2013 to 2017.