[Objective] The spatiotemporal evolution characteristics of cultivated land non-agriculturalization in Fujian Province from 1980 to 2020 were systematically studied based on county scale area, and the spatial distribution, migration law, and aggregation characteristics of cultivated land non-agriculturalization in Fujian Province were revealed in order to provide a basis for the protection and sustainable utilization of cultivated land resources in Fujian Province.[Methods] Based on land use data of Fujian Province in 1980, 1990, 2000, 2010, and 2020, a center of gravity model and spatial autocorrelation methods were used to analyze the spatio-temporal evolution characteristics of the non-agriculturalization of cultivated land in Fujian Province.[Results] ① The cultivated land resources in Fujian Province were mainly distributed in a belt-shaped manner along the southeastern coast, and the area of cultivated land decreased year by year over time. ② The spatial distribution of cultivated land non-agriculturalization in Fujian Province exhibited significant geographical differences, and the degree of cultivated land non-agriculturalization has been increasing, gradually expanding from the southeast coastal area to the entire province. The high grade areas of cultivated land non-agriculturalization were mainly distributed in the southeast coastal area and the northwest area, with the conversion of cultivated land mainly into construction land, forest land and grassland. ③ The gravity center of cultivated land non-agriculturalization in Fujian Province deviated from the geometric center, and its distribution was uneven. The center of gravity of cultivated land non-agriculturalization was mainly located to the southeast of the geometric center, shifting first to the southwest, then to the southeast, and then to the northwest. The degree of cultivated land non-agriculturalization in the southeastern part of Fujian Province was still greater than in the northwestern part of the province. The overall trend of the spatial pattern of cultivated land non-agriculturalization shifted over time from the southeastern part to the northwestern part of the province. ④ Cultivated land non-agriculturalization in Fujian Province showed a positive spatial correlation and a deepening spatial clustering. The spatial changes of the "high-high" and "low-low" agglomeration played a dominant role in the evolution of the spatial autocorrelation of cultivated land non-agriculturalization in Fujian Province, with the "high-high" agglomeration shifting from the southeast to the northwest, and the "low-low" agglomeration shifting from the southeast to the northwest. The "high-high" agglomeration shifted from the southeastern region to the northwestern region, and the number of "low-low" agglomeration decreased.[Conclusion] The degree of non-agricultural land in Fujian Province is deepening, the degree of spatial aggregation is strengthening and the center of gravity of non-agricultural land is gradually moving to Northwest China. In order to ensure food security and take into account economic development, reasonable measures should be taken to protect cultivated land and realize sustainable development of cultivated land resources.