[Objective] Based on the land use data of five periods in Jiangxi Province from 2000 to 2020, the spatial and temporal evolution characteristics of habitat quality and the main factors of regional habitat quality change were studied, in order to provide references for land use planning and regional ecosystem service optimization in Jiangxi Province.[Methods] The InVEST habitat quality assessment model was used to measure the spatial habitat quality level in Jiangxi Province. The global Moran's I index was used to analyze the spatiotemporal correlation of habitat quality. The Geodetector model was used to capture the dominant factors affecting habitat quality.[Results] ① From 2000 to 2020, the overall habitat quality in Jiangxi Province was at a relatively high level, but showed a slow downward trend. ② The habitat quality in Jiangxi Province had a high spatial positive correlation, and the spatial distribution differences gradually increased. ③ In the natural socio-economic driving system, the proportion of forest land in land use type factors was the main controlling factor affecting habitat quality. The interactions between each driving factor were nonlinear enhancements, with the greatest interaction observed between the distance from construction land and the proportion of cultivated land.[Conclusion] The overall habitat quality level in Jiangxi Province was relatively high, with significant spatial distribution differences. Habitat quality level was mainly influenced by land use types.