[Objective] The changes of soil biomes and organic matter in typical forest park area of eastern Zhejiang were studied to lay a foundation for improving soil structure and increasing soil organic matter content in this area. [Methods] The forest land, orchard, vegetable and wasteland land utilization way, by wet sieving method big aggregate (> 2 mm) > middle aggregate (0.25-2 mm) > micro aggregate (0.053-0.25 mm) > powder clay (< 0.053 mm) in the content of organic carbon, microbial biomass carbon and nitrogen content, the influence of soil aggregate stability and microbes will provide a scientific basis for the optimization of the management. [Results] ①The results showed that the distribution trend of the components of the aggregates of different soil particle sizes was consistent under different land use patterns. 0 to 10 cm soil under different land use ways mean weight diameter (MWDS) and geometric mean diameter (GMD) were higher than 10-20 cm soil layer, 0 to 10 cm and 10-20 cm soil MWDS and GMD roughly characterized by forest land > wasteland > orchard > vegetables, forest land and wasteland of no significant difference (p > 0.05), orchards and vegetable no significant difference (p > 0.05), illustrate that different ways of land use on soil aggregate stability influence mainly in the surface soil. ② Soils under different land use ways each grain size trends of organic carbon content is consistent, basic performance is: the big aggregate > middle aggregate > micro aggregate > powder clay, and 0 to 10 cm soil layer soil of each particle size distribution of organic carbon content were higher than 10-20 cm soil layer, 0 to 10 cm and roughly 10-20 cm soil organic carbon content of forest land > wasteland > orchard > vegetables, significantly higher than that of forest land and wasteland orchards and vegetable (p < 0.05), orchards and vegetable no significant difference (p > 0.05). ③ Soils under different land use modes of each particle size of microbial biomass carbon and nitrogen variation trend is consistent, basic performance is: the big aggregate > middle aggregate > micro aggregate > powder clay, and 0 to 10 cm soil layer soil microbial biomass carbon and nitrogen content were higher than 10-20 cm soil layer, 0 to 10 cm and 10-20 cm soil microbial biomass carbon and nitrogen content is generally characterized by forest land > wasteland > orchard > vegetables, significantly higher than that of forest land and wasteland orchards and vegetable (p < 0.05), orchards and vegetable no significant difference (p > 0.05) . [Conclusion] On the whole, the organic carbon, microbial biomass carbon and nitrogen of the forest land and wasteland aggregate were higher than that of the orchard and vegetable land, indicating that the forest land and wasteland were more conducive to the accumulation of soil organic carbon and could promote soil carbon sequestration.