[Objective] A logical system of “connotation interpretation, identification and evaluation, and regulation and resolution” of land use conflicts was constructed to explore the spatial types and pattern conflicts in Yancheng City, Jiangsu Province from 2010 to 2022 and to provide references for the optimization of territorial spatial layout and marine ecological environment protection. [Methods] Taking Yancheng City as an example, the spatial type and pattern conflict index were constructed based on the scale characteristics of conflicts and the landscape ecology model based on the land profit data for 2010, 2015, and 2022, respectively, to identify the spatial units and intensities of land use conflicts at the three scales of Yancheng City: City, district or county, and grid. It also revealed the conflict of interest in the social and economic background, putting forward the regulation and optimization measures. [Results] ① Owing to the strict protection of ecological space, spatial development would mainly occupy agricultural space. Coastal areas and counties would present more complex conflicts of interest due to the promotion of coastal economic development strategy and ecological protection. ② The spatial pattern conflicts in Yancheng City from 2010 to 2022 were mainly found in the western inland region and the coastal land of Dafeng District, which were related to the high ecological risk of land use change driven by lake wetland protection and coastal economic development. ③ The Yancheng City should focus on coordinating the main contradictions between economic development and basic farmland protection, and there was a need to standardize the conflict of interests on land use for the smooth promotion of coastal economic development strategy. [Conclusion] From 2010 to 2022, the ecological space of Yancheng City had been controlled strictly. The pressure of cultivated land protection in the city was relatively larger, and the contradiction of coastal development was relatively complicated. The conflict between economic development and agricultural protection should be solved in the whole city, and the conflict between port economy, aquaculture development and beach wetland protection should be coordinated in the coastal area.