[Objective] The changes of soil biomes and organic matter in typical forest park areas of Eastern Zhejiang Province were studied to lay a foundation for improving soil structure and increasing soil organic matter content in this area. [Methods] Detecting aggregate (0.25—2 mm), micro aggregate (0.053—0.25 mm), big aggregate (>2 mm), and powder clay (<0.053 mm) in the content of organic carbon, microbial biomass carbon and nitrogen content, the influence of soil aggregate stability, and microbes using wet sieving method under different land utilization way of forest land, orchard, vegetable land, and waste land to provide a scientific basis for formation pattern and regulation of soil organic carbon pool. [Results] ① The results showed that the distribution trend of the components of the aggregates of different soil particle sizes was consistent under different land-use patterns. The mean weight diameter (MWDS) and geometric mean diameter (GMD) of 0—10 cm soil under different land use ways were higher than those of the 10—20 cm soil layer. The MWDS and GMD of both types of soil were roughly characterized by forest land > wasteland > orchard > vegetable land; forest land and wasteland showed no significant difference (p>0.05), and neither did orchards and vegetable land (p>0.05); this demonstrates the influence of different ways of land use on soil aggregate stability, mainly in the surface soil. ② For soils under different land-use patterns, each grain size trend of organic carbon content is consistent, and the basic performance is big aggregate > micro aggregate > middle aggregate > powder and clay; for the 0—10 cm soil layer, each particle’s size distribution of organic carbon content was higher than that of 10—20 cm soil layer, and the organic carbon content of forest land > wasteland > orchard > vegetable land for the 0—10 cm and 10—20 cm soil is significantly higher than that of forest land and wasteland orchards and vegetable land (p<0.05), whereas orchards and vegetable land show no significant difference (p>0.05). ③ Soils under different land-use patterns of each particle size of microbial biomass carbon and nitrogen variation trend are consistent, and the basic performance is big aggregate > micro aggregate > middle aggregate > powder and clay; for the 0—10 cm soil layer, the soil’s microbial biomass carbon and nitrogen content were higher than those of the 10—20 cm soil layer, and the microbial biomass carbon and nitrogen content of 0—10 cm and 10—20 cm soil is generally characterized by forest land > wasteland > orchard > vegetable land, significantly higher than that of forest land and wasteland orchards and vegetable land (p<0.05), whereas orchards and vegetable land show no significant difference (p>0.05). [Conclusion] Overall, the organic carbon, microbial biomass carbon, and nitrogen of the forest land and wasteland aggregate were higher than those of the orchard and vegetable land; thus, forest land and wasteland were more conducive to the accumulation of soil organic carbon and could promote soil carbon sequestration.