Abstract:[Objective] The corridor-pinch point-barrier pattern of the wilderness network in Wenshan Prefecture, Yunnan Province and its spatiotemporal evolution characteristics were systematically characterized in order to provide a scientific basis for formulation of territorial spatial development, ecological zoning and control, and wilderness conservation strategy in Wenshan Prefecture. [Methods] Wilderness mapping was used to analyze the wilderness landscape patterns in 2005, 2015, and 2025. Morphological spatial pattern analysis（MSPA） was employed to identify wilderness sources, the minimum cumulative resistance model（MCR） was used to calculate the minimum resistance surfaces of wilderness sources, and circuit theory was applied to extract wilderness corridors, pinch points, and barrier points. [Results] From 2005 to 2025, both high-quality wilderness and wilderness sources in Wenshan Prefecture showed a phased change of “first decreasing and then increasing”. The area of high-quality wilderness decreased from 17 327.40 km2 to 14 393.89 km2 and then increased to 21 717.11 km2, while the source area decreased from 4 737.96 km2 to 4 612.14 km2 and then increased to 6 330.15 km2. The network connectivity structure fluctuated synchronously with changes in sources. The total corridor length decreased from 11 698.80 km to 6 532.42 km and then increased to 22 324.80 km. However, the area of barrier points continued to expand, with an increase of 355.54 km2, indicating an expansion of high-resistance areas and a growing risk of connectivity degradation. [Conclusion] Overall, the wilderness network in Wenshan Prefecture exhibits a phased evolution of initial contraction followed by restoration. Ecological protection should adopt a hierarchical strategy of “source priority, corridor protection and bottleneck management”: strictly protect core sources; prioritize corridors with high movement potential as the main restoration routes; and implement focused remediation and control in areas where pinch points and barrier points are concentrated to reduce corridor blockage risks and stabilize regional connectivity patterns.