Abstract:[Objective] The effects of tartaric acid(TA) as an amendment on the respiration of reclaimed soil were investigated in order to reveal the dynamic variation patterns of soil respiration under TA addition and its interactive effects with environmental factors. [Methods] Taking the reclaimed soil from the Panyi mine reclamation area in northwestern Huainan City, Anhui Province as the research object, a gradient-controlled experimental design was adopted. Gradients of TA application rate and concentration were systematically set, coupled with dual environmental factor gradients of temperature and soil moisture content, so as to conduct longterm continuous in-situ monitoring of the respiration rate of reclaimed soil. [Results] ① Compared with the control group(TA application rate = 0), TA addition at a rate lower than 675 mg/kg promoted the respiration of reclaimed soil. In contrast, TA addition at a rate higher than 675 mg/kg exhibited an inhibitory effect on respiration of reclaimed soil.② When soil temperature and moisture content changed, the daily cumulative CO2 respiration of reclaimed soil showed a trend of first rapidly increasing and then decreasing during the incubation period after TA addition. The promoting effect of TA on respiration of reclaimed soil followed the order of temperature groups: 35 ℃ TA > 25 ℃ TA > 15 ℃ TA, and the order of moisture content groups: 25% TA > 15% TA > 35% TA. At 35 ℃, the average daily cumulative respiration over the 20-day incubation was 11.03 times higher than that of the CK group, while at 25% soil moisture content, it was 5.14 times higher than that of the CK group.③ Redundancy analysis(RDA) indicated that TA concentration was the key factor affecting the respiration of reclaimed soil, followed by temperature. [Conclusion] Appropriate addition of TA can significantly increase the CO2 respiration rate of reclaimed soil, drive the dynamic changes of soil nutrient content, and optimize the physicochemical and biological properties of reclaimed soil, thereby contributing to the long-term stable utilization of reclaimed soil.