[Objective] Areas of ecological space conflict in the Hefei metropolitan area were identified， and collaborative strategies were proposed to provide references for regional ecological protection and economic development. [Methods] Ecological source areas and corridors were extracted using morphological spatial analysis and a minimum resistance model to construct an ecological security pattern. Coupling the suitability of supply and the scarcity of demand， the reasonable range of urban and agricultural spaces were evaluated， and key areas of non-ecological space were identified； The superposition analysis method was adopted to calculate the ecological space conflict index. [Results] ① Central and southern regions exhibited superior ecological connectivity， contrasting with severe fragmentation in northern areas. Ecological sources and corridors demonstrated distinct differentiation of more in the south and less in the north， with core sources aggregating in the southern hilly regions. ② Non-ecological spaces displayed concentric zonation： high development suitability and demand in the central zones， transitional characteristics in the suburbs， and lower values in peripheral areas. ③ Spatial conflicts manifested as heterogeneity of higher at the center， and lower alonge the edge with high and higher conflict zones （24.21% of the area） concentrated in urban-rural transition areas， reflecting ecological sensitivity and contradictions in development demand. Medium-low conflict zones （75.79%） occupied buffer regions with favorable ecological conditions and limited human disturbance. ④ In ecological space conflict zones， a dual governance mechanism featuring ‘ecological red lines enforcement’ and ‘adaptive non-ecological space management’ should be established， incorporating conditional access policies and dynamic adaptation strategies to reconcile ecological integrity with development needs. [Conclusion] The ecological space conflicts in metropolitan areas present a gradient differentiation feature of form core area to periphery. The coordinated allocation and differentiated governance of ecological and non-ecological spaces are the main paths for achieving ecological protection and high-quality development.