[Objective] The spatial trade-offs of multifunctional cultivated land in mountainous and hilly regions of southwest China under the dual constraints of rapid urbanization and ecological conservation were analyzed, in order to provide scientific support for decision-making regarding sustainable land resource utilization and the alleviation of human-land conflicts. [Methods] Taking Chongqing City as the research area, a spatial function evaluation system was constructed for the three characteristics (production, life and ecology) of cultivated land. Methods such as the entropy weight method, comprehensive index evaluation method, coupling coordination degree model and K-means clustering analysis were adopted to explore the spatiotemporal evolution characteristics of multi-functionality and the coupled coordination relationship of cultivated land in Chongqing City from 2013 to 2022. [Results] ① The overall production function of cultivated land in Chongqing City showed the characteristics of lower in the east and higher in the west, larger concentration and smaller dispersion. This was mainly concentrated in the western part of Chongqing City on the periphery of the main urban area, while the ecological function formed high-value areas in the ecological corridors along the Wuling Mountains, Daba Mountains and the Yangtze River. ② The coupling and coordination degree of the three living space functions of cultivated land in Chongqing City significantly improved from 2013 to 2022, transforming from ‘low efficiency and imbalance’ to ‘initial coordination’. ③ Cultivated land in Chongqing City can be divided into an agricultural optimization transition zone (16.22%), agricultural production vitality zone (40.54%), and ecological life conservation zone (43.24%). The utilization patterns of cultivated land in different functional zones showed clear spatial differentiation characteristics. [Conclusion] The multi-functionality of cultivated land in Chongqing City showed significant regional differentiation characteristics in temporal and spatial dimensions. Overall, it had transformed from ‘low efficiency and imbalance’ to ‘initial coordination’, but the imbalance in development among regions still exists. Greater attention should be attached on the multi-functional optimization of cultivated land in mountainous cities, regional territorial spatial planning, and the formulation of sustainable development policies.