Nowadays eco-environmental water demand is becoming a hot spotiven the field of water resource and re-lated fields. The relationship between eco-environmental water demand and water resource was analyzed. Demand evaluating index system for the eco-environmental water included one object layer,four guide layers and 20 index-es. The evaluating measures were set up,which enriches the theory of eco-environmental water demand. In addi-tion,based on the eco-environmental water demands of Anji County,the clemands of six counties in different loca-tions of Zhejiang Province were calculated and evaluated. The conclusion is that eco-environmental water demand for Tonglu County,Tiantai Countyand Zhuji County are on excellent level,and for Yongkang County,Ruian County and Longyou County,on good level. The bottleneck factors of each city can be found accordingly.