This study was conducted based on the 26 year positionfixed experiments of non-calcareous fluro-aquic soil,during two years of 2002 and 2003,12 treatments were designed: CK,N_1,N_2,M_1,M_1N_1,M_1N_2,M_2,M_2N_1,M_2N_2,NPK,NP and NK.Numbers of soil microbe and soil animal numbers in winter wheat and summer corn rotations were measured.Results indicated that both the amount of bacteria,epiphyte and actionmyces sharply and the amounts of acarina,collembolan and nematode were increased by application of organic fertilizer and the combined application of inorganic fertilizer with organic fertilizer for a longtime.Soil animal collembolan and nematode were significantly related to soil organic material and available phosphorous and available nitrogen.Acarina was significantly related to soil organic phosphorous,available potassium and available nitrogen.It indicated that they can be used as the biological indices of soil fertilization properties and quality.