The spatial difference of nature-ecology leads to the difference of ecological functions and directly influences the regional ecological security. Resorted to the evaluation of sub-area by the nature-ecology,the difference of ecological functions may be distinguished. Supported with GIS and by adopting the regionaliza tion method of eco-environmental restriction,indexes,such as mountain area,water density,etc,are selected and 41 counties are regarded as the basic units to be evaluated. After evaluation,regional ecological secur ity pattern is constructed with 4 ecological functional zones,7 ecological functional corridors,and some eco logical functional patches in the area along the Yangtze River in Anhui Province. Finally,some policies and suggestions abouthow to constructregional ecological security pattern are putforward.