The concept of ecosystem health is introduced in highway roadside ecosystem by taking Dabao Highway (from Dali City to Baoshan City) and Sixiao Highway (from Simao City to Xiaomengyang Town) in the longitudinal range-gorge region as examples. Distance index,coordination index,and health index are adopted and ecosystem health state on highway roadside is assessed. Then the effect caused by highway construction on the roadside ecosystem health is analyzed. Results indicate that highway construction has dramatic effects on highway roadside ecosystem health,with affected scope mostly within 200 m. The nearer to the road,the harder the effect is. The affecting degrees on highway roadside ecosystem health under different landforms caused by the two selected highways are obviously different. The degree of Dabao Highway is higher than that of Sixiao highway,except the ecosystem within 0—50 m to road on mountainside section. The compared result between different landforms shows the order of mountaintopmountainside.