With the increased investments in infrastructure in China,tunnel construction is strengthened,its technology is improved,and its scale is increasingly large.These have some significant influences on ecological environment at the same time.The connotation of tunnel project and its effects on ecosystem are discussed and the effects of tunnel project,including physical effect,chemical effect,biological effect,and social effect,are analyzed.Ecological tunnel project is a logical choice to maintain ecosystem health.Ecological strategies of tunnel project are discussed from the aspects of survey and design,construction,and benefit operation.Ecological tunnel project can alleviate the interference and damages to structure and functions of ecosystem and the threats to ecosystem health.This study may provide some references to the coordination of tunnel construction with environmental protection.