In order to solve the problem that there is no suitable platform to estimate ecological baseflow byusing different methods,a software to estimate ecological baseflow with friendly interface,simplicity ofoperation,and integrated 12hydrological and hydraulics methods is developed by using Visual Basic 6.0.Itnot only simplifies the estimation for ecological baseflow,but also easily makes comparison among differentmethods.A case study in the Weihe River basin was carried out by using this software.Results show thatecological baseflows estimated by using different methods are significantly different.Therefore,the averageof various methods,13.7m3/s,is selected as the ecological baseflow and the result of Texas method is regarded as the monthly ecological baseflow in the Weihe River. This software provides a great support for thedevelopment of the dynamic estimation of ecological baseflow in the future.