This paper used the latest research results of ecosystem services value method and map overlay of UIS method to assess environmental impact of new general land use planning of Yangzhou City.The research results indicated that ecological services value of Yangzhou City ascended after a short period of declining during the planning period,while the municipal district and Baoying County presented a descending trend.Ecological services value of Yizheng,Uaoyou and Jiangdu City presented a consistent ascending trend;Most planned constructive land was distributed in non-sensitive and low-sensitive areas,and 3.62%constructive land was distributed in middle-sensitive areas,and only 0.36%constructive land was distributed in high-sensitive areas,so the distribution of constructive land in the plan scheme had relatively good ecological suitability.This paper put forward feasible preventive and alleviative measures aiming at minimizing the negative impacts which may be caused by the implementation of the plan.In the context of strict implementation of the plan and the carrying out of relative preventive and alleviative measures,this plan is feasible from the perspective of environmental protection and sustainable development.Using ecosystem services value method and map overlap of UIS method to assess environmental impacts of general land use planning is scientific and feasible.