Groundwater resources are the most important source to life and industrial water use in Southwest China karst-mountains and provide an important guarantee for the all-side sustainable development of social, economic and ecological construction. The present status and characteristics of groundwater pollution were analyzed based on the analysis of groundwater resources use in Southwest China karst-mountains. Results indicate that the groundwater pollution in Southwest China karst-mountains mainly comes from four aspects: urban economic activities, industrial and mining exploitation, the "three wastes" emission, agricultural non-point source pollution and rural living garbage pollution. On this basis, several measures were discussed and put forward: strengthening investigation and monitoring of groundwater in karst region; strengthening water environmental protection publicity and education and improving villagers' water environmental protection consciousness; adjusting industrial structure and promoting rural waste treatment; developing organic ecological agriculture; and promoting the prevention and controlling countermeasures of rural ecological environment and groundwater pollution controlling. Prevention is recognized to be the key to control karst groundwater pollution.