Impacts of cement content on soil biological activities in vegetation-concrete substrate are discussed. By taking vegetation-concrete substrate covered by common revetment herb as the study object, the soil biological activity index(SBAI) is calculated based on experimental data of urease, invertase, alkaline phosphatase, microbial biomass carbon and microbial biomass nitrogen. Results indicate that adding cement can reduce soil biological activities and the SBAI was at a higher level with low cement content. Under the same gradient of cement content, the SBAI of unmanaged samples is obviously lower than managed samples covered by Cynodon dactylon and Medicago sativa L., which shows that plants can improve the level of soil biological activities to a certain extent.